| Group | Club/Team Name | Team ID | Head Coach |
| D21 | Beach United FC - 01 Beach United FC - Fusion |
03022-001AG2U-1268 | Sh'Nell Briscoe |
| D22 | Bluffton Rush Soccer Club - 01 Bluffton Rush 2012 Girls |
03040-001AG2U-0001 | Nicholas Lewis |
| D23 | Cainhoy Athletic Soccer Club CASC 12 Hurricanes |
03006-001AG2U-2381 | Brent Wood |
| D24 | Charleston City FC CCFC U12 Girls |
03014-001JG2U-0038 | Alison Davis |
| D25 | Tormenta FC Academy - 01 TFC 12G Ibis Statesboro |
03035-001AG2U-0020 | Ayomikun Fajemisin |
| D26 | Mt. Pleasant SC - 01 MPRD Black Panthers |
03007-001RG2U-0007 | Kevin Kollegger |
| D27 | James Island Youth SC JIYSC 2012 Girls Black Tzavlas |
03001-001AG2U-0007 | Michael Tzavlas |
| D28 | James Island Youth SC JIYSC 2012 Girls Black Hedley |
03001-001AG2U-0008 | Ryan Hedley |
| D29 | Daniel Island SA DISA U12 Gators Jr. Academy |
03030-001AG2U-0002 | Erik Cobb |
| D210 | Tormenta FC Academy - 01 TFC 12G Ibis II |
03035-001AG2U-0019 | Nicolas Boegemann |
| D211 | Charleston Soccer Club LLC CSC 12 Girls Navy North |
03034-001AG2U-0049 | Damon Dixon |
| D212 | Florence Soccer Association - 01 FSA 2012 Girls White |
03043-001JG2U-0015 | Faith Allen |
| D213 | Charleston United SC - 01 Charleston United 12 Girls Blue |
03002-001RG2U-0013 | Jurgen Wagner |